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. 2006 Nov 13;27(3):963–974. doi: 10.1128/MCB.00795-06

FIG. 2.

FIG. 2.

Analysis of the mutant Mod(mdg4) proteins in vitro. (A) Western analyses of yeast extracts carrying different Mod(mdg4) mutants. The panel shows the expression Mod(mdg4) proteins fused to GAL4 binding domain detected with GAL4 antibodies. In the lower panel, the same filter was stripped and reprobed with anti-tubuline antibodies. (B) Western blot analyses of E. coli-expressed and purified mutant Mod(mdg4) proteins. The native PAGE and sodium dodecyl sulfate-PAGE of the same proteins are shown in the upper and lower panels, respectively. Experimental details are described in Materials and Methods. (C) Interaction of the Mod(mdg4) mutants with Mod(mdg4)-67.2 by GST pull-down assay. The interactions of mutant Mod(mdg4) proteins with wild-type Mod(mdg4)-67.2 were visualized by Western blot analysis using the anti-His tag monoclonal antibodies. All results were reproduced in three independent experiments.