FIG. 5.
Localization of mutant Mod(mdg4) proteins on polytene chromosomes of the y2scD1; mod(mdg4)u1/mod(mdg4)u1 line. Salivary glands were dissected from third-instar larvae. The upper panel shows 4′,6′-diamidino-2-phenylindole (DAPI)-stained chromosomes. The lower panel represents the localization of Mod(mdg4) on polytene chromosomes; the white arrows indicate the Mod(mdg4) bands. The mutant Mod(mdg4) proteins were detected using a polyclonal antibody against the Mod(mdg4)-67.2 isoform and Cy3-conjugated secondary antibodies.