TABLE 2.
Identification of the second domain required for dimerization of Mod(mdg4)-67.2a
| Interacting proteins | Strength of interaction |
|---|---|
| Mod(mdg4)ΔBTB, Mod(mdg4)ΔQ | +++ |
| Mod(mdg4)ΔBTB, Mod(mdg4)1-273 | − |
| Mod(mdg4)1-273, Mod(mdg4)1-273 | +++ |
| ModD33N, Mod(mdg4)1-273 | − |
| ModH46D, Mod(mdg4)1-273 | − |
| ModD33N/H46D, Mod(mdg4)1-273 | + |
| ModD33N/R47Q, Mod(mdg4)1-273 | + |
| Mod(mdg4)GAF, Mod(mdg4)1-273 | ++ |
| ModR47Q, Mod(mdg4)1-273 | +++ |
| ModR47Q1-273, ModR47Q1-273 | +++ |
| ModR47Q1-273, Mod(mdg4)1-273 | +++ |
| ModD33N/H46D1-273, ModD33N/H46D1-273 | − |
| ModD33N/H46D1-273, Mod(mdg4)1-273 | + |
| ModD33N/R47Q1-273, ModD33N/R47Q1-273 | − |
| ModD33N/R47Q1-273, Mod(mdg4)1-273 | + |
| Mod(mdg4)234-610, Mod(mdg4)234-610 | ++ |
| Mod(mdg4)317-610, Mod(mdg4)317-610 | ++ |
| Mod(mdg4)390-610, Mod(mdg4)390-610 | − |
| Mod(mdg4)ΔDD, Su(Hw)MID | +++ |
| ModD33N/H46DΔDD, Su(Hw)MID | +++ |
| Mod(mdg4)ΔDD, Mod(mdg4)ΔDD | +++ |
| ModD33N/H46DΔDD, Mod(mdg4)ΔDD | + |
| ModD33N/H46DΔDD, Mod(mdg4) | + |
| ModD33N/H46DΔDD, ModD33N/H46DΔDD | − |
a
The relative strength of the two-hybrid interaction was similar in both directions. The GAL4AD was on the C terminus of the fused proteins. No growth occurred after transformation with single plasmids, indicating that interactions between the proteins are required for expression of the reporter genes (data not shown). Equivalent expression of the chimeric proteins in yeast was confirmed by immunoblotting with GAL4 BD or AD monoclonal antibodies (Fig. 2A and data not shown). Designations are as defined for Table 1.