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. 2006 Nov 27;27(4):1370–1379. doi: 10.1128/MCB.01486-06

FIG. 5.

FIG. 5.

Visual cycle gene expression in Rdh12−/− versus wild-type mice. (A) Transcript abundance assayed using quantitative real-time PCR, normalized to Hprt expression and shown as an x-fold increase compared to the wild-type abundance. (B) Western analysis of protein expression in whole-eye protein extracts, using the following antibodies and dilutions: anti-Rdh5, 1:1,000; anti-Rpe65, 1:5,000; anti-Lrat, 1:1,000; anti-Cralbp, 1:1,000; anti-rhodopsin, 1:1,000; anti-Nrl, 1:1,000; anti-arrestin, 1:1,000; and anti-Gapdh, 1:4,000.