FIG. 2.
TD immune responses are defective in Cd19Cre/+ Mapk1flox/flox mice. Mice were immunized with TNP-Ficoll (A) or TNP-KLH in alum (B and C). Sera were analyzed by ELISA for TNP-specific IgM and IgG3 (A) or TNP-specific IgM, IgG1, IgG2b, and IgG3 (B) by using TNP25-BSA as a capturing antigen. A secondary immunization with TNP-KLH without adjuvant in PBS was given 42 days after primary immunization (C). Collected sera from four mice were measured for high-affinity anti-TNP IgG1 by using TNP3-BSA as a capturing antigen. For all panels, each time point represents the average and standard error of the mean for four mice.