Skip to main content
. 2006 Dec 4;27(4):1394–1406. doi: 10.1128/MCB.00736-06

FIG. 3.

FIG. 3.

Loss of DDB-1 and CUL-4 is associated with enlarged blast cells with excessive DNA content. (A) DIC image of lateral hypodermis of wild-type L2-stage larvae, and ddb-1(tm1769), cul-4(gk434), and cul-4(gk434);cki-1(RNAi) arrested L2-stage larvae (3 days posthatching). Se, seam cell nuclei; H, hyp7 cell nuclei. (B) Seam cell nuclei of wild-type L2-stage larvae, and ddb-1(tm1769), cul-4(gk434) and cul-4(gk434);cki-1(RNAi) arrested L2-stage larvae (3 days posthatching), stained with DAPI (blue) and anti-CDT-1 (green). Anti-AJM-1 staining (red), which highlights gap junctions (37), was overlaid to mark seam cell boundaries. (C) Seam cell nuclei of wild-type and arrested ddb-1(RNAi) L3-stage larvae, stained with DAPI (blue), anti-CKI-1 (green), and anti-CYE-1 (red). Scale bar, 10 μm.