FIG. 2.
RNA polymerase II association at transcribed genic and nongenic β-globin sequences. ChIP was performed using antibodies specific to the pol II N-terminal region (N-20; Santa Cruz), the initiation/elongation Ser5P CTD form of pol II (H14; Covance), and the elongation/termination Ser2P CTD form of pol II (H5; Covance). Values obtained by real-time qPCR were normalized to input by using the comparative CT method and may be rescaled, i.e., divided or multiplied by a factor, as indicated, to accommodate individual antibody efficiencies and allow for direct comparison of the patterns of pol II localization. The results are the averages of three RNA preparations.