FIG. 4.
H3K4, K9, and K36 trimethylation patterns in the human β-globin locus. The me3 patterns for H3K4, K36, and K9 from Fig. 3A, B, and C are plotted together and in some instances were rescaled (see the legend to Fig. 2) to allow for direct comparison of the methylation patterns obtained with different antibodies. ChIP was performed using antibodies to H3. All values are normalized to input using the comparative CT method (n = 3), and the final values obtained were multiplied by 3 in order to compare the pattern of H3 distribution to those of histone trimethylation. For additional control reactions where the scale has not been changed, including error bars, see Fig. S2 in the supplemental material.