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. 2006 Dec 4;27(4):1531–1543. doi: 10.1128/MCB.00629-06

FIG. 6.

FIG. 6.

βMyHC dβNRE-S element confers Pur-dependent expression on a minimal TK promoter. (A) Schematic representation of wild-type (TK-3x dβNRE-S wt) and mutant (TK-3x dβNRE-Sm1) heterologous reporter genes. (B) C2C12 myoblasts were cotransfected with various combinations of either TK-3x dβNRE-S wt or TK-3x dβNRE-Sm1 reporter genes and Purα, Purβ, or Purα and Purβ expression plasmids and allowed to differentiate. Forty-eight hours later, C2C12 myotube cellular extracts were collected and assayed for luciferase activity. Data are reported as luciferase-normalized relative light units (RLU) (firefly/Renilla) and are expressed as the mean ± standard error (n = 8). *, P < 0.0001; all comparisons are against TK-3x-dβNRE-S wt activity. ns, not significant.