FIG. 2.

The npa2-1 mutation confers a slow growth phenotype and is synthetically lethal with the rsa3 null allele. Strains IVY251 (npa2-1), carrying the plasmid YCplac33-NPA2, and YMD3-2D (Δrsa3) were crossed, the resulting diploid was sporulated, and tetrads were dissected. Complete tetrads were streaked on YPD plates and restreaked on 5-FOA-containing plates to counterselect YCplac33-NPA2. A representative tetratype tetrad is shown on a YPD plate (top half) or on a 5-FOA containing plate (bottom half). Plates were incubated at 30°C for 4 days.