FIG. 2.
Complex formation between ectopic ERα and Fe65 in HeLa cells. (A) Cells were transfected as described in the legend to Fig. 1A, with or without 0.3 μg of HA-Tip60, and treated for 48 h. Luciferase activity was determined and normalized with β-galactosidase activity. (B) Cells were transfected with 2 μg of pLENhERα, with or without 4 μg of HA-Fe65, and treated with EOH or 10−8 M E2 for 72 h. Immunoprecipitation (IP) analyses were performed, followed by immunoblotting (IB) with the indicated antibodies.