FIG. 3.

Complex formation between ERα and APPct mediated through Fe65. (A) HeLa cells were transfected with 4 μg of Gal4-APPct and pLENhERα, with or without 4 μg of HA-Fe65, and treated for 72 h. Coimmunoprecipitations were performed with the indicated antibodies. (B) ERα protein, either alone (lanes 3 and 4) or together with Fe65 protein (lanes 1 and 2), was produced by in vitro transcription-coupled translation in the absence or presence of E2. GST pull-down assays were performed, and the precipitated proteins were detected by immunoblotting. An image of Coomassie blue (C.B.) staining was included to show that similar amounts of GST and GST-APPct were used for the pull-down assays.