FIG. 3.

Binding of Ime1-HA and Nps1-TAP to the IME2 URS1 site. The strains used were WMY11-D (A), WHK40-D (B), WMY12-D (C), and WMY13-D (D). The cells were harvested at the indicated times, divided into two portions, and processed for ChIP analysis and immunoblotting. The amount of immunoprecipitated DNA obtained by anti-HA antibody (A, C, and D) or IgG-Sepharose (B) was determined by PCR with primer pairs directed against IME2-URS1, calculated as described in the legend to Fig. 2B, and shown as a relative amount with the value of the YPD-grown cells (Y) of each strain taken as 1. As a control, a primer set for the telomere of chromosome VI-R (TEL) (A, C, and D) or HTA1 ORF (B), on which no binding of RSC occurs (19), was used. The ratio of the URS1 signal to the TEL signal or to the HTA1 signal of untagged samples was almost equivalent to that of input samples. The values are averages of three independent experiments. Ime1-HA and Nps1-TAP were detected with anti-HA and anti-Nps1 antibody, respectively.