TABLE 1.
Gene-specific primers and probes used for real-time PCRa
| Gene | Primer | Probe |
|---|---|---|
| BMP-2 | F: CCCGCGTGCTTCTTAGAC | CATGGTCGACCTTTAG |
| R: GAAGCAGCAACGCTAGAAGACA | ||
| C3 | F: GGAGCCAGTGGACATCTGAGA | TCTCCATCAAGATTCC |
| R: CCCTCCTTATCTGAGTTGAATTCCT | ||
| Dkk-1 | F: GGGCTGTGTTGTGCAAGACA | TTCTGGTCCAAGATCT |
| R: GGTGCACACCTGACCTTCTTTAA | ||
| c-Jun | F: CCGGTGGGCAGTCTGAAG | CCGAGTTCTTGGCGCG |
| R: GTCGGGCGACGTGAGAAG | ||
| Osteocalcin | F: GCTGCGCTCTGTCTCTCTGA | AAGCCCAGCGGCC |
| R: TGCTTGGACATGAAGGCTTTG | ||
| Runx2 | F: AAGGTTCAACGATCTGAGATTTGTG | ACGAGGCAAGAGTTT |
| R: GTGAAGACGGTTATGGTCAAGGT | ||
| Wnt1 | F: AACCGCCGCTGGAACTG | TTCGGCAAGATCGTC |
| R: GCGAAGATGAACGCTGTTTCTC | ||
| β-Gal | F: GGCTTACGGCGGTGATTTTG | ATCGTTCGGCGTATCGC |
| R: CAAAGACCAGACCGTTCATACAGAA |
a
F and R denote forward and reverse primers, respectively.