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. 2006 Dec 4;27(4):1455–1466. doi: 10.1128/MCB.01369-06

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Copyright © 2007, American Society for Microbiology

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FIG.1. — 14-3-3τ^−/− animals do not survive embryonic development. A. Male and female 14-3-3τ^+/− animals were mated, and embryonic offspring were analyzed for genotype by PCR at specific time points after fertilization. B. 14-3-3τ^−/− embryos die by embryonic day 14. Genetic analysis of embryos obtained from 14-3-3τ^+/− × 14-3-3τ^+/− matings at various embryonic stages. C. Deciduae containing 14-3-3τ^−/− embryos are smaller than heterozygous deciduae. Deciduae from 14-3-3τ^+/− × 14-3-3τ^+/− matings were isolated from adult females at 8 and 11 dpc. In several cases at 11 or 12 dpc, deciduae containing 14-3-3τ^−/− embryos were partially resorbed or were dead (see bottom right panel). D. 14-3-3τ^−/− embryos are hypomorphic and exhibit developmental delay at 9 and 10 dpc. Embryos were carefully dissected from deciduae and were photographed under a dissecting microscope. Embryos were placed in formalin and were again imaged. E. Cardiac development is delayed but is otherwise normal in 14-3-3τ^−/− embryos. Formalin-fixed embryos were embedded in paraffin, sectioned with a microtome, and stained with hematoxylin and eosin. A transverse section of a paraffin-embedded 9-dpc 14-3-3τ^−/− embryo is depicted in the figure. Am, amnion; En, endocardium; FG, foregut diverticulum; Myo, myocardial layer of ventricle; NF, neural folds.

FIG.1. — 14-3-3τ^−/− animals do not survive embryonic development. A. Male and female 14-3-3τ^+/− animals were mated, and embryonic offspring were analyzed for genotype by PCR at specific time points after fertilization. B. 14-3-3τ^−/− embryos die by embryonic day 14. Genetic analysis of embryos obtained from 14-3-3τ^+/− × 14-3-3τ^+/− matings at various embryonic stages. C. Deciduae containing 14-3-3τ^−/− embryos are smaller than heterozygous deciduae. Deciduae from 14-3-3τ^+/− × 14-3-3τ^+/− matings were isolated from adult females at 8 and 11 dpc. In several cases at 11 or 12 dpc, deciduae containing 14-3-3τ^−/− embryos were partially resorbed or were dead (see bottom right panel). D. 14-3-3τ^−/− embryos are hypomorphic and exhibit developmental delay at 9 and 10 dpc. Embryos were carefully dissected from deciduae and were photographed under a dissecting microscope. Embryos were placed in formalin and were again imaged. E. Cardiac development is delayed but is otherwise normal in 14-3-3τ^−/− embryos. Formalin-fixed embryos were embedded in paraffin, sectioned with a microtome, and stained with hematoxylin and eosin. A transverse section of a paraffin-embedded 9-dpc 14-3-3τ^−/− embryo is depicted in the figure. Am, amnion; En, endocardium; FG, foregut diverticulum; Myo, myocardial layer of ventricle; NF, neural folds.