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. 2006 Nov 13;27(4):1348–1355. doi: 10.1128/MCB.00658-06

FIG. 5.

FIG. 5.

Conditional expression of COM1 induces slow growth rates in SYO-1 cells. (A) Expression of COM1 in SYO-1 cells that conditionally express COM1 using the Tet-Off system. Clones #8 and #10 are shown with doxycycline (+) and at 48 h after doxycycline withdrawal (−). Empty vector-transfected (Vec.) SYO-1 cells (clones #4 and #9) are also shown as controls. Expression of COM1 in HeLa cells that stably express COM1 (clones #4 and #12) and empty vector-transfected HeLa cells (clone #2) are also shown. COM1 expression was analyzed by RT-PCR, which was performed for 30 cycles. (B) Expression of SYT or SYT-SSX in SYO-1 cells that conditionally express COM1 using the Tet-Off system. RNAs were extracted from SYO-1 cells as described in the legend to panel A. Expression levels of SYT and SYT-SSX were analyzed by RT-PCR. The overexpression of COM1 showed no effects for the expression levels of SYT or SYT-SSX in SYO-1 cells. (C) Analysis of cell growth of SYO-1 cells that conditionally express COM1 or empty vector after the withdrawal of doxycycline [induction (+)] for 11 days. (D) Analysis of cell growth of HeLa cells that stably express COM1 or empty vector for 8 days.