FIG. 4.

Genetic organization of the ropy eps gene cluster of plasmid pEPS352 of L. lactis subsp. cremoris Ropy352. Restriction sites relevant for pGh9:ISS1-mediated subcloning are shown. The symbol @ indicates the replication origin used for recovery of the EcoRI fragment. Large block arrows with diagonal dashes indicate partial ORFs. The large block arrows filled with dots correspond to novel genes described in the text. The pGh9:ISS1 insertion site is indicated. Putative RBS-promoter sequences for the epsN, epsM, and epsU genes and the EPS352 gene operon are indicated with black flags. The corresponding plasmid of the pGh9:ISS1-mediated subcloned fragment is indicated below the arrows. The small block arrows with vertical lines are the two fragments of epsH as described in the text.