TABLE 2.
Properties and similarities of novel enzymes encoded by pEPS352 eps
| pEPS352 gene | Predicted protein sizea | pI of protein | G+C content (%) | Putative translation start sequenceb | Similar polypeptide(s) | % Identity/similarity | Organismc | Putative function of gene product |
|---|---|---|---|---|---|---|---|---|
| epsM | 331/38.6 | 9.61 | 27 | GGAGtataaaattgATG | EpsG | 35/54 | S. thermophilus (1) | Glycosyltransferase |
| CpsI | 35/54 | S. thermophilus (2) | Glycosyltransferase | |||||
| EpsH | 35/52 | S. thermophilus (1) | Glycosyltransferase | |||||
| EpsI | 33/55 | S. thermophilus (3) | Glycosyltransferase | |||||
| epsN | 329/38.3 | 8.69 | 30.2 | AGGAGtagaataaagagATG | Eps7F | 94/96 | S. thermophilus (4) | Glycosyltransferase |
| EpsH | 34/52 | S. thermophilus (1) | Glycosyltransferase | |||||
| EpsV | 32/55 | S. thermophilus (5) | Glycosyltransferase | |||||
| Eps9I | 32/53 | S. thermophilus (6) | Glycosyltransferase | |||||
| epsO | 235/27.8 | 7.74 | 30.2 | GAGAAgatATG | Eps7I | 86/91 | S. thermophilus (4) | Putative glycosyltransferase |
| EpsQ | 41/61 | L. lactis subsp. cremoris (7) | Putative glycosyltransferase | |||||
| Eps10 | 41/59 | S. thermophilus (8) | Putative glycosyltransferase | |||||
| WciT | 38/56 | S. pneumoniae (9) | Putative glycosyltransferase | |||||
| epsP | 364/42.2 | 9.89 | 26.9 | GGAAaataattactATG | Eps7H | 78/82 | S. thermophilus (4) | Putative repeating-unit polymerase |
| Orf7B | 78/79 | S. thermophilus (4) | Putative repeating-unit polymerase | |||||
| CpsIVH | 22/41 | S. agalactiae (10) | Putative repeating-unit polymerase | |||||
| Eps4N | 19/40 | S. thermophilus (11) | Putative repeating-unit polymerase | |||||
| epsU | 472/53.5 | 9.61 | 30.2 | GGAGGgttattaATG | CpsU | 97/98 | S. thermophilus (12) | Glycosyltransferase |
| EpsU | 97/98 | S. thermophilus (5) | Glycosyltransferase | |||||
| EpsI | 97/98 | S. thermophilus (1) | Glycosyltransferase | |||||
| Eps7M | 96/97 | S. thermophilus (4) | Glycosyltransferase |
a
Number of amino acids/molecular mass in kilodaltons.
b
The lactococcal 16S rRNA sequence of the 3′ end is 3′-UCUUUCCUCC-5′ (12). Uppercase letters indicate putative RBS, and boldface indicates the start codon.