TABLE 2.
Initial cell count/ml | Lag time (min) for growth of cells transferred from:
|
|||
---|---|---|---|---|
Liquid to plate
|
Liquid to liquid
|
|||
Expo phase | Stat phase | Expo phase | Stat phase | |
108 | 180 | 180 | 0 | 60 |
107 | 180 | 180 | 0 | 60 |
106 | 180 | 180 | 0 | 60 |
105 | 180 | 180 | 0 | 60 |
104 | 180 | 180 | 20 | NDb |
103 | 180 | 180 | 20 | ND |
E. coli MG1655 cells were grown in LB, collected in the exponential (Expo) phase (OD600 = 0.5) or stationary (Stat) phase (8 h), and plated on LBA or grown on LB. Growth on plates was followed as described in Materials and Methods. Experiments were repeated at least four times, and standard deviations were always below 10%.
ND, not determined.