TABLE 6.
Related pathway, amino acid, and ion (m/z) | Model prediction | Measured value |
---|---|---|
Pyruvate synthesis route | ||
Ala | ||
M0 | 0.02 | 0.03 ± 0.01 |
M1 | 0.98 | 0.98 ± 0.01 |
M2 | 0 | 0 ± 0 |
Val | ||
M0 | 0.02 | 0.05 ± 0.01 |
M1 | 0.96 | 0.94 ± 0.01 |
M2 | 0 | 0 ± 0 |
Serine oxidation route | ||
Ser | ||
M0 | 0.24 | 0.21 ± 0.03 |
M1 | 0.76 | 0.79 ± 0.03 |
M2 | 0.0 | 0 ± 0 |
Gly | ||
M0 | 0.25 | 0.34 ± 0.03 |
M1 | 0.75 | 0.66 ± 0.03 |
M2 | 0 | 0 ± 0 |
TCA cycle | ||
Asp | ||
M0 | 0.78 | 0.75 ± 0.04 |
M1 | 0.12 | 0.14 ± 0.02 |
M2 | 0.10 | 0.10 ± 0.01 |
Glu/Gln | ||
M0 | 0.89 | 0.86 ± 0.03 |
M1 | 0.09 | 0.07 ± 0.02 |
M2 | 0.01 | 0.03 ± 0.02 |
PP pathway | ||
Phe | ||
M0 | 0.01 | 0.01 ± 0 |
M1 | 0.13 | 0.09 ± 0.03 |
M2 | 0.42 | 0.36 ± 0.05 |
M3 | 0.42 | 0.52 ± 0.08 |
His | ||
M0 | 0.23 | 0.18 ± 0.05 |
M1 | 0.76 | 0.75 ± 0.06 |
M2 | 0.01 | 0.04 ± 0.02 |
The flux rate distribution listed in Fig. 3 (for the carbon-limited condition) was used for model prediction.