TABLE 6.

Results for flux distribution reliability test: predicted and measured fragment mass [M-57]⁺ distributions of key amino acids when 1-¹³C-labeled lactate medium was used for shake flask culture (n = 2)^a

Related pathway, amino acid, and ion (m/z)	Model prediction	Measured value
Pyruvate synthesis route
Ala
M0	0.02	0.03 ± 0.01
M1	0.98	0.98 ± 0.01
M2	0	0 ± 0
Val
M0	0.02	0.05 ± 0.01
M1	0.96	0.94 ± 0.01
M2	0	0 ± 0
Serine oxidation route
Ser
M0	0.24	0.21 ± 0.03
M1	0.76	0.79 ± 0.03
M2	0.0	0 ± 0
Gly
M0	0.25	0.34 ± 0.03
M1	0.75	0.66 ± 0.03
M2	0	0 ± 0
TCA cycle
Asp
M0	0.78	0.75 ± 0.04
M1	0.12	0.14 ± 0.02
M2	0.10	0.10 ± 0.01
Glu/Gln
M0	0.89	0.86 ± 0.03
M1	0.09	0.07 ± 0.02
M2	0.01	0.03 ± 0.02
PP pathway
Phe
M0	0.01	0.01 ± 0
M1	0.13	0.09 ± 0.03
M2	0.42	0.36 ± 0.05
M3	0.42	0.52 ± 0.08
His
M0	0.23	0.18 ± 0.05
M1	0.76	0.75 ± 0.06
M2	0.01	0.04 ± 0.02

^aThe flux rate distribution listed in Fig. 3 (for the carbon-limited condition) was used for model prediction.