TABLE 2.
Nucleotide sequences of primers used for PCR analyses in this study
| Primer | Sequence (5′-3′) | Target | Reference(s) |
|---|---|---|---|
| A | AAGTGGCGTTGCTTTGTGAT | yehV insertion site region | 27 |
| B | AACAGATGTGTGGTGAGTGTCTG | ||
| A | AAGTGGCGTTGCTTTGTGAT | Bacteriophage-yehV right junction | 27 |
| E | GATGCACAATAGGCACTACGC | ||
| F | CACCGGAAGGACAATTCATC | Bacteriophage-yehV left junction | 27 |
| B | AACAGATGTGTGGTGAGTGTCTG | ||
| C | AGGAAGGTACGCATTTGACC | wrbA insertion site region | 27 |
| D | CGAATCGCTACGGAATAGAGA | ||
| C | AGGAAGGTACGCATTTGACC | Bacteriophage-wrbA right junction | 27 |
| G | ATCGTTCGCAAGAATCACAA | ||
| H | CCGACCTTTGTACGGATGTAA | Bacteriophage-wrbA left junction | 27 |
| D | CGAATCGCTACGGAATAGAGA | ||
| A | AAGTGGCGTTGCTTTGTGAT | Variant bacteriophage-yehV right junction | 27; this study |
| E2 | CAGGGAATCAATCGCAGTTT | ||
| 595 | CCGAAGAAAAACCCAGTAACAG | stx2-antiterminator Q gene junction | 33, 17 |
| Q933 | CGGAGGGGATTGTTGAAGGC | ||
| TirF | TCATGTTCAGCCGTTTATCG | tir | This study |
| TirR | AAGCTCAAGAGTTGCCCATC | ||
| A | AAGTGGCGTTGCTTTGTGAT | Bacteriophage-yehV right junction (∼8 kb) | 27; this study |
| R1 | GCAGATGATGAGCGTGATTG | ||
| FimH460F | CTTATGGCGGCGTGTTATCTA | FimH mannose-binding pocket flanking N135K | Shaikh et al., submitted |
| FimH460R | GCCAGTAGGCACTACCACATC | ||
| stx2c-F919 | CTGAACAGAAAGTCACAGTYTTTA | stx2c-specific region | This study |
| stx2c-R1100 | GGCCACTTTTACTGTGAATGTATC | ||
| O-F163 | ATGCGCAAGACATACGGATTC | Stx2c bacteriophage O gene | This study |
| O-R695 | TGCACAAACGCCCTGACATA | ||
| Q-F69 | GGGCGCATGGGTTTATTCA | Stx2c bacteriophage Q gene | This study |
| Q-R389 | ACTTCCCGTCGGCAGGTTG | ||
| mdh-F41 | AGGCGCTTGCACTACTGTTA | mdh | This study |
| mdh-R875 | AGCGCGTTCTGTTCAAATG |