TABLE 1.
Effect of different treatments on the percent binding of anti-TDmAb to the nuclei of irradiated sporozoites
| Sporozoite treatment | % Antibody binding (mean ± SD)a | Sporozoite morphology |
|---|---|---|
| Fixed in PFA or methanol and air dried | 10.3 ± 4.0 | Excellent |
| Microwaved for 2 min, fixed in PFA or methanol, and air dried | 28.7 ± 7.5 | Damaged |
| Heated in water bath at 70°C for 5 min, fixed in PFA or methanol, and air dried | 63.3 ± 6.5 | Damaged |
| Air dried overnight at room temp and then fixed in methanol | 83.7 ± 7.9 | Varies from good to damaged |
a
Percent antibody binding indicates the number of sporozoites with anti-TDmAb+, DAPI+ nuclei (n = 300 observations per sporozoite treatment).