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Mass spectrum profiles of the TMS-derivatized metabolite detected at the retention time of 21.58 min: (A) from a culture fluid of S. aciditrophicus grown with unlabeled crotonate; (B) from a culture fluid of S. aciditrophicus grown with [U-13C]crotonate; and (C) from a TMS-derivatized cyclohexane carboxylate authentic standard. The dashed arrows (panel C) represent the fission sites of the different fragments of cyclohexane carboxylate.
