TABLE 1.
Effects of growth pH on the total fatty acid composition of L. monocytogenes 10403S
| Growth pH | Buffer | % Total fatty acids (mean ± SD)a
|
ACCLc (mean ± SD) | ||||
|---|---|---|---|---|---|---|---|
| Anteiso | Iso | Anteiso/iso | Straight | Branched | |||
| 7.0 | Tris | 75.16 ± 3.63 | 20.2 ± 4.72 | 3.86 ± 0.95 | 2.85 ± 0.14 | 95.37 ± 1.09 | 15.46 ± 0.16 |
| 8.0 | Tris | 80.77b ± 0.26 | 14.8b ± 0.59 | 5.44b ± 0.23 | 2.04b ± 0.21 | 95.64 ± 0.35 | 15.39 ± 0.08 |
| 8.5 | Tris | 84.30b ± 0.39 | 12.6b ± 0.43 | 6.69b ± 0.22 | 1.55b ± 0.33 | 96.91b ± 0.66 | 15.52 ± 0.01 |
| 9.0 | Tris | 77.17 ± 0.41 | 20.8 ± 0.58 | 3.70 ± 0.12 | 1.38b ± 0.07 | 98.04b ± 0.16 | 15.64 ± 0.03 |
| 7.0 | Phosphate | 76.69 ± 0.39 | 21.0 ± 0.36 | 3.62 ± 0.06 | 2.13 ± 0.61 | 97.86 ± 0.61 | 15.77 ± 0.02 |
| 6.0 | Phosphate | 71.97b ± 1.18 | 25b ± 1.29 | 2.93b ± 0.19 | 3.22b ± 0.48 | 96.56 ± 0.58 | 15.75 ± 0.02 |
| 5.5 | Phosphate | 72.55b ± 0.74 | 24b ± 0.58 | 3.08b ± 0.10 | 3.44b ± 0.16 | 96.06b ± 0.15 | 15.73 ± 0.03 |
| 5.0 | Phosphate | 80.24b ± 0.68 | 16b ± 0.12 | 5.10b ± 0.08 | 3.02 ± 0.37 | 95.97 ± 0.55 | 15.68 ± 0.04 |
a
Values are from three independent experiments.
b
Statistically different (P < 0.05) from the control cultures (pH 7.0).
c
ACCL, average carbon chain length, given by the equation [Σ(FAP × C)]/100, where FAP is the percentage of each fatty acid and C is the number of carbon atoms in the chain.