FIG. 5.

(Left panel) Relationship between optimal growth pressure and loop elongation in the genera Shewanella and Photobacterium. (Right panel) Detection of ribotype composition in various strains by semiquantitative PCR. The assay was performed for 25 cycles as described in Materials and Methods (lane 1, 25-bp ladder; lane 2, PE36; lane 3, PT48; lane 4, PT64; lane 5, F1A; lane 6, KT99; lane 7, 3TCK; lane 8, 1230; lane 9, DSJ4; lane 10, SS9; lane 11, 25-bp ladder). Because of chimera formation, in Photobacterium strains only the short-short, the short-long, and the long-short ribotypes could be detected with the general primers. The long-long ribotype had to be amplified with specific primers.