TABLE 3.
Comparison of coding sequences of recombinant genes used in this study
| Gene product | Gene length (nucleotides) | GC content (%) | No. of rare codonsa |
|---|---|---|---|
| scFv-phOx | 777 | 55.0 | 12 |
| GM-CSF | 378 | 57.7 | 10 |
| IFN-α2b | 495 | 48.4 | 13 |
| IFN-α2bS | 495 | 53.6 | 0 |
a
Rare codons are as follows. scFv-phOx: arginine, CGA (3 codons), AGA (4 codons), and AGG (1 codon); leucine, CTA (1 codon); isoleucine, ATA (1 codon); and proline, CCC (2 codons). GM-CSF: arginine, CGG (2 codons) and AGA (1 codon); leucine, CTA (1 codon); and proline, CCC (6 codons). IFN-α2b: arginine, AGG (4 codons) and AGA (5 codons); leucine, CTA (1 codon); isoleucine, ATA (1 codon); and proline, CCC (2 codons).