TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant property(ies) | Source or reference |
|---|---|---|
| Strains | ||
| DH5α | thi-1 recA hsdR17 lac | BRL Life Technologies |
| MC4100 | araD139 Δ(argF-lac) U169 rpsL150 relA flbB5301 ptsF25 deoC1 | R. Hengge-Aronis |
| BL21 (DE3) | High-level expression using T7 RNA | G. Anderluh |
| polymerase-based expression systems | ||
| AB1133 | Sensitive to all colicins | B. Bachman |
| UL98 | hlyA cnf1 usp iroN iucD fyuA fimH traT | 26 |
| Apr Tcr Nalr Cmr Kmr Str | ||
| UL114 | K1 iucD iroN fyuA usp traT Apr Cipr | 26 |
| UL31 | hly cnf1 papC papG sfa/foc iucD iroN usp Cmr Nalr | 26 |
| UL173 | K5 iroN iucD fimH traT Apr Sxtr Cipr | 26 |
| Plasmids | ||
| pColK-K235 | Wild-type plasmid | 18 |
| pUC19 | Apr cloning vector | 36 |
| pBR322 | Apr Tcr cloning vector | 3 |
| pET8c | Apr expression vector | 25 |