TABLE 2.
Oligonucleotide primers used in this study
| Oligonucleotide primer | Nucleotide sequencea | Positionb |
|---|---|---|
| ColK1 | TCACTCGAGCATGGCTAAAGAAC | 2527-2555 |
| TAAGTGGATATGGACC | ||
| ColK2 | CCACGCGTTAAATCCCTAACAA | 4172-4143 |
| CTCATTAAGTTTGCTC | ||
| cka1 | CAGAGGTCGCTGAACATGAAAC | 3263-3284 |
| cka2 | CTCAGCTAAACGCTTCTCTGCTT | 3336-3314 |
| P1 | TAACCGGGAAGGGGCGAGAGTTCT | 6268-6291 |
| P2 | GAATGAACTGGACGCCGACG | 5737-5718 |
| P3 | TTCTGAACTGACGGGGATCGC | 4591-4611 |
| P4 | TGAAACACGCCAGTTCGCCC | 1899-1918 |
| P5 | GCTTCCAGGGCCGCTCTTCCT | 7154-7134 |
| PK1 | GGCGTATTTTCCCCGGTTTC | 673-692 |
| PK2 | TGGTGCCAGCCAGTCTGCTC | 594-575 |
| PK3 | GGGCGAACTGGCGTGTTTCA | 1918-1899 |
| PK4 | ACACCCTTGTTGCTGCCGGA | 2234-2215 |
| PK5 | TCCGGCAGCAACAAGGGTGT | 2215-2234 |
| PK6 | AGCTGCGTGGCCTTTTCCGG | 5216-5235 |
| PK7 | TCCGCCTTTCTCCCTTTGGG | 5054-5035 |
| PK8 | TACAGCGCCCCTTTCAGCCT | 6881-6862 |
| PK9 | AGGAAGAGCGGCCCGGAAGC | 7134-7154 |
| PK10 | AGGCTGAAAGGGGCGCTGTA | 6862-6881 |
| PK11 | CCCCTTCACGGATTTGCAGC | 7531-7550 |
| mob1 | GGCAGTGGTCCGGTTGAT | 6230-6248 |
| mob2 | TGCAGCCCGTAATGGTGA | 6630-6612 |
| rna1 | AGGATCTTCTTGAGATCCTT | 4632-4652 |
| rm2 | TATCCACAGAATCAGGGG | 5291-5273 |
| rom1 | AAGCGGGCCATGTTAAGG | 5774-5792 |
| rom2 | AATCAACCGGACCACTGC | 6248-6230 |
a
Nucleotides in boldface correspond to introduced XhoI and MluI restriction sites.
b
Numbers indicate positions on plasmid pColK-K235.