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. 2006 Dec 1;73(3):808–814. doi: 10.1128/AEM.00399-06

TABLE 2.

Molecular beacon sequences and characteristics

Beacon Sequencea Fluorophore Quencher dGb (kcal/mol) Tmc (°C)
Subgroup I
    Beacon 1 CTGGGGCCAGCGAGCTCTCCTCCCCAG FAMd Dabcyl −1.26 65.1
Subgroup II
    Beacon 2 CTCGCGCAGGAAACGTATGGCGATGAGACGCGAG HEXe Dabcyl −1.55 65.2
Subgroup III
    Beacon 3 ACGGCAAGCGGGTGCAGTTCCTGCCGT CY-5f BHQ2g −1.7 68.2
Subgroup IV
    Beacon 4 CCCAGGCAAATAAAGCAGTCACTGTTCCTGGG Texas-Red Dabcyl −0.83 61.1
a

Subgroup-specific sequences are in boldface.

b

dG, predicted minimum free energy.

c

Tm, predicted melting temperature.

d

FAM, 6-carboxyfluorescein.

e

HEX, hexachloro-6-carboxyfluorescein.

f

CY-5, indodicarbocyanine.

g

BHQ-2, black hole quencher 2.