TABLE 2.
Recombinant production of EPA and/or DHA using pfa genes from Shewanella pneumatophori SCRC-2738, Moritella marina MP-1, and Photobacterium profundum SS9a
| Source and combination of pfa genes of S. pneumatophori SCRC-2738 | Source and combination of pfa genes
|
Production of EPA and/or DHA | Reference | |
|---|---|---|---|---|
| M. marina MP-1 | P. profundum SS9 | |||
| pfaABCDEb | EPA | 20 | ||
| pfaABCDc plus pfaEd | EPA | 20 | ||
| pfaABCDc | pfaEe | EPA | 17 | |
| pfaABCDf plus pfaEg | DHA | 19 | ||
| pfaBCDEh | pfaABCDf | EPA and DHA | 18 | |
| pfaEd | pfaA*BCDi | None | 17 | |
| pfaA*BCDi plus pfaEg | None | 17 | ||
| pfaABCDj plus pfaEk | None | 1 | ||
a
This table is modified from data reported previously in reference 18.
b
pfa genes are harbored in pEPAΔ1.
c
pfa genes are harbored in pEPAΔ1,2,3.
d
The pfaE gene is harbored in pSTV::pfaE.
e
The pfaE gene is harbored in pETSTV::pfaE.
f
pfa genes are harbored in pDHA3.
g
The pfaE gene is harbored in pET21::pfaE.
h
pfa genes are harbored pEPAΔ5.
i
pfa genes are harbored in pDHA2, where pfaA* is inactive.
j
pfa genes are harbored in pFOS8E1.
k
PPTase genes from Bacillus subtilis and Shewanella sp. strain SC2A were used.