FIG. 2.

Insulin increases recruitment of the chromatin remodeling complexes to the promoters of insulin target genes. (A) Western blot analysis of the protein level of the nuclear active form of ADD1/SREBP1c in 3T3-L1 adipocytes with (+) or without (−) insulin (100 nM) for 1 h. (B and C) ChIP analyses of the ADD1/SREBP1c promoter (B) and PEPCK promoter (C). 3T3-L1 adipocytes were incubated with or without insulin (100 nM). The amount of each PCR product was quantitated for the graph. For each experiment, 1% of input was used. α, anti.