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Effect of additional Hsc82 mutations on cochaperone interaction in the presence of AMP-PNP. WT and mutant His-Hsc82 complexes were isolated and analyzed as described in the legend to Fig. 3 except that lysate was supplemented with 5 mM AMP-PNP. In all cases, WT or mutant His-Hsc82 was the only Hsp90 protein present in the cell.
