TABLE 1.
Hsc82 mutants used in this work
| Mutation | Location of mutation | Growth phenotype of hsc82 hsp82 straina
|
Reference(s) (protein) | |
|---|---|---|---|---|
| 30°C | 37°C | |||
| None | ++++ | ++++ | 2 | |
| T22I | ATPase | − | − | 23 (Hsp82-T22I) |
| E33A | ATPase | − | − | 24, 25 (Hsp82-E33A) |
| D79N | ATPase | − | − | 24, 25 (Hsp82-D79N) |
| T101I | ATPase | − | − | 23 (Hsp82-T101I) |
| A107N | ATPase | ++++ | +++ | 29 (Hsp82-A107N) |
| W296A | Middle | ++ | − | 20 (Hsp82-W300A) |
| G309S | Middle | ++++ | − | 23 (Hsp82-G313S) |
| F325A | Middle | ++++ | +++ | This study |
| F345A | Middle | ++++ | − | 20 (Hsp82-F349A) |
| N373A | Middle | ++++ | ++ | 20 (Hsp82-N377A) |
| R376A | Middle | − | − | 20 (Hsp82-R380A) |
| E377K | Middle | − | − | 23 (Hsp82-E381K) |
| Q380A | Middle | ++++ | − | 20 (Hsp82-Q384A) |
| S481Y | Middle | ++++ | − | 15 (Hsp82-S485Y) |
| L487S | Middle | ++++ | − | 10; this study |
| T521I | Middle | ++++ | − | 15 (Hsp82-T525I) |
| A583T | C terminus | ++++ | − | 23 (Hsp82-A587T) |
| I588A M589A | C terminus | ++++ | − | This study |
| L647S L648S | C terminus | ++ | − | 40; this study |
| F660A | C terminus | ++++ | ++++ | This study |
| ΔMEEVD | C terminus | ++++ | ++++ | 16 (Hsp82-ΔMEEVD) |
a
WT growth. Growth defects correspond to an approximate 10-fold (+++) and 100-fold (++) reduction in colony numbers observed upon serial dilation growth assays.