FIG. 2.

Deletion of SLM1 confers supersensitivity to myriocin. (A) Isogenic wild-type, slm1Δ, and slm2Δ mutant strains were grown to stationary phase in YPD medium, and serial dilutions of cultures were spotted on YPD plates containing either drug vehicle alone or the indicated concentrations of myriocin. (B) Addition of exogenous PHS (10 μM) to YPD plates containing 0.5 μg/ml myriocin rescues growth of the slm1Δ mutant. All plates were incubated at 30°C and photographed after 3 days.