Table 1.

Cleavage rate and dissociation constants for L-21 ScaI mutant ribozymes

Ribozyme	Product binding*			Substrate cleavage†
	Kd, nM	Rel.	ΔΔG, kcal/mol	kcat, min−1	Rel.
Wild-type	11 ± 2	1.0	0.00	0.0450  ±  0.0008	1.0
1X	92 ± 3	8.4	1.32	0.00240  ±  0.00014	18
2X	50 ± 9	4.5	0.94	0.0195  ±  0.0007	2.3
3X	12 ± 3	1.1	0.05	0.0425  ±  0.0006	1.1

^*Average of at least three independent CCCUCU-binding experiments. 

^†Average of at least two independent CCCUCdTAAAAA-cleavage experiments. Reported errors are the standard deviation of the sample.