FIG. 4.

Myocardin induced cardiac muscle-specific gene expressions in fibroblast 10T1/2 cells in the presence of SUMO-1/PIAS1. (A) RT-PCR was performed on total RNA from 10T1/2 cells transfected with specified expression vectors alone or in various combinations as indicated. GAPDH expression level was measured as a control (Cont). Comparable data were obtained from two separate experiments. Ca, cardiac. (B) Sarcomeric α-MHC was detected in 10T1/2 cells transfected with various specified factors as indicated using MF20 in immunofluorescence staining assays. V5 antibody was used to detect myocardin-positive cells. The data shown represent three independent assays. (C) The percentage scores of 100 cells among the V5-positive are shown.