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. 2006 Nov 13;27(2):777–787. doi: 10.1128/MCB.01460-06

FIG. 2.

FIG. 2.

RNAi reveals that TbMP18 is essential. (A) Northern blot showing the ∼1-kb TbMP18 mRNA and the ∼0.5-kb double-stranded RNA (dsRNA) transcript from uninduced (−) or 24-h-induced (+) RNAi cells. In this experiment and all subsequent experiments, additional clones gave analogous results. (B) Cell growth profile of two independent, clonal RNAi cell lines (RNAi) and control 29.13 cells (Co) following Tet addition. (C and D) Western blots showing TbMP18 and a load control protein (hsp or lip) from the control cells (Co) and RNAi cells, in rapid cell extracts (26) prepared on day 3 (d3) and day 6 (d6) postinduction (C) and in traditional mitochondrial extracts (18, 41) prepared on day 4 (d4) postinduction (D). The protein amount analyzed is indicated below the extract designation, with 1x equal to 3 μg. Dashes on these and subsequent protein gels indicate the position of the examined protein. Quantitation of the gels (see Materials and Methods) is shown below the lanes (N represents values not significantly above the background level); for the load control protein, the values represent the relative intensities, while for TbMP18, they represent the relative intensities after correcting for the signal from the load control protein.