FIG. 3.

Retention of other editing proteins upon depletion of TbMP18. Results of Western analyses of the indicated proteins (left) from control 29.13 cells and RNAi cells, assessed from rapid cell extracts prepared at day 3 (d3) (A) and day 6 (d6) (B) of induction and from traditional extracts prepared at day 4 (d4) of induction (C) are shown. (D) Load controls for the different gels using either hsp or lip antibody, as indicated. For each antibody and type of extract, the gels were run and blotted together. The numbers below the extract designation indicate the relative amounts of extract protein loaded onto the gel, part of a larger analyzed range of extract dilutions. The ≅ columns of panels A through C indicate the approximate remaining abundance of each protein, relative to the corresponding load control band, as determined for TbMP18 in Fig. 2C and D (quantitation not shown for panel D). These numbers represent a combination of the values from quantitation of these bands and of some additional Western blotting analyses and are rounded to the nearest 0.05.