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TbMP18 is not critical for the U-deletional and U-insertional ligation activities. Assessments of U-deletional ligation (A), precleaved U deletion (3′-U-exo plus ligation) (B), and precleaved U insertion (TUTase plus ligation) (C) used the substrates diagramed below, as described in the text. (In panel A, 1× equals 1.2 μg protein.) The fully edited −3U lig and +2U lig products are indicated.
