FIG. 8.

Assessment of precleaved editing in traditional extracts and their glycerol gradient fractions. (A and B) Precleaved U deletion activity was assessed as described for Fig. 7B except for use of traditional extracts (1× equals 1.2 μg protein) (A) or the indicated glycerol gradient fractions (B). (Control experiments [data not shown] indicate that ligation in the RNAi gradient fractions in panel B was not limited by inefficient ligase activation.) (C) Precleaved U insertion activity was assessed as described for Fig. 7C. To save space, in panels A through C, the blank middle regions of these gels are not shown. Assessment of the individual 3′-U-exo and TUTase activities (not shown) gave results similar to those shown in the lower gel regions of panels A and B and of panel C, respectively. (D) Basic TUTase activity was assessed as described for Fig. 6C. The TbMP108 TUTase sediments in fractions 12 and higher fractions (not shown).