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. 2006 Nov 13;27(2):568–578. doi: 10.1128/MCB.00731-06

FIG. 3.

FIG. 3.

A surviving orc2-1 clone with extensively shortened Chr XII shows a partially suppressed ts phenotype. (A) ts phenotypes of surviving orc2-1 clones. Cells of wild-type (NOY408-1b), orc2-1 (YNV1), and two surviving orc2-1 clones (SvC1 and SvC2) were grown in YPD at 23°C. Cells were serially fivefold diluted with distilled water, spotted onto YPD plates, and incubated at 23°C, 26°C, and 28°C for 2.5 days. (B) Sizes of Chr XII in surviving orc2-1 clones. Chromosomes in wild-type, orc2-1, SvC1, and SvC2 cells were analyzed by PFGE as described in Materials and Methods. The left panel for each strain shows the ethidium bromide-stained chromosome profile, and the right panel is an autoradiogram obtained after Southern hybridization with probe 058 for Chr XII. Positions of Chr XII are indicated by triangles.