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. 2006 Nov 13;27(2):568–578. doi: 10.1128/MCB.00731-06

FIG. 7.

FIG. 7.

Effects of rdnΔ30 on the origin activities of rDNA ARSs in Chr XII. (A) Two-dimensional gel electrophoresis of replication intermediates of chromosomal fragments containing rDNA ARSs from wild-type (150 copies of the rDNA) and rdnΔ30 (30 copies) strains. DNA was prepared from NOY408-1bf (fob1Δ), NOY408-1bf30 (fob1Δ rdnΔ30), YNV2 (fob1Δ orc2-1), and YNV15 (fob1Δ orc2-1 rdnΔ30) cells growing at 23°C, digested with NheI, and subjected to 2D gel electrophoresis followed by Southern hybridization using an rDNA-specific probe, as described in Materials and Methods. Brackets show Y-fork intermediates paused at a region encoding 5S rRNA. (B) Increased origin activities of rDNA ARSs in wild-type and orc2-1 cells with reduced copy numbers of rDNA. Origin activities in NOY408-1bf (fob1Δ), NOY408-1bf30 (fob1Δ rdnΔ30), YNV2 (fob1Δ orc2-1), and YNV15 (fob1Δ orc2-1 rdnΔ30) cells were determined for rDNA ARSs as described in the legend for Fig. 6. The thick bars indicate the average origin activities from two independent experiments, and the values expressed are relative to that obtained with NOY408-1bf. Error bars represent standard deviations.