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. 2006 Oct 30;27(2):481–496. doi: 10.1128/MCB.00463-06

FIG. 2.

FIG. 2.

Cellular localization of HA-tagged WT and mutant Comm in muscles. Longitudinal and cross-section confocal images of HA-tagged Comm (WT and mutants) expressed in muscle 12 or 13 of third-instar larvae that were costained with the plasma membrane marker concanavalin A (ConA). (A and B) Expression of Comm(WT) in UAS-comm+(WT)/24B-GAL4 larvae (A), with higher magnification shown in panel B, revealing vesicular expression of Comm(WT). (C and D) Expression of Comm(2PY→AY) in UAS-comm2PY→AY/24B-GAL4 larvae (C) or embryos (D). (E) Expression of Comm(10K→R) in UAS-comm10K→R/24B-GAL4 larvae. Note the accumulation of mutant Comm [Comm(2PY→AY) and Comm(10K→R)] at the plasma membrane of the muscle, while Comm(WT) accumulates in intracellular vesicles. Scale bars, 10 μm.