FIG. 7.

Replication timing of the Ebf1 locus is shifted to later replication in Pax5-deficient cells. (A) Replication timing of the early-replicating α-globin gene locus, the late-replicating X141 locus, and the Ebf1 locus in wild-type (wt) and Pax5-deficient pro-B cells is depicted. (B) Schematic overview of the Ebf1 locus. The locations of the primer pairs used for analysis of the replication timing are indicated. (C) In wild-type pro-B cells, Ebf1 replication peaked at the first quarter of S phase (S1). (D) Pax5-negative pro-B cells display later replication (S2) of the Ebf1 locus. (E) An even later replication was observed in ES cells, where Ebf1 is silent. A domain of later replication “centered” at Ebf1 was observed in Pax5-negative and ES cells, which extended to an area of more than 1 Mb, spanning the 37324131I11Rik and EpsinR genes that are active in both pro-B and ES cells.