FIG. 2.
Matrix ATP levels and conditional mutants of the import motor complex determine the protease resistance of translocation intermediates. Experiments and quantifications were performed as described in the legend to Fig. 1. The reactions were performed with conditional-mutant mitochondria from the ssc1-2 (A), tim44-8 (B), and pam16-1 (C) strains. The temperature-sensitive phenotype was induced by incubation of the isolated mitochondria for 15 min at 37°C prior to the import reaction. The protease-resistant processed form of b2(167)Δ-DHFR was quantified, and the amount obtained in the absence of protease was set to 100%.