FIG. 4.

Disruption of the murine Sulf2 gene. (A) Schematic representation of the gene trap insertion into the Sulf2 gene between exon 5 and exon 6. (B) TaqMan analysis of Sulf1 and Sulf2 mRNA in total RNA derived from the lungs and kidneys of wild-type (WT), Sulf2^+/−, and three independent Sulf2^−/− mice. Sulf gene expression was normalized to the expression of hypoxanthine phosphoribosyltransferase and presented as a relative quantitative score relative to Sulf expression in wild-type lungs. (C) RNA in situ analysis of Sulf gene expression in wild-type and Sulf2^−/− homozygous E14.5 embryos. Sulf2 message was not detected in Sulf2^−/− embryos, and the Sulf1 expression pattern does not appear to change. The signal sequence (S), splice acceptor (SA), transmembrane domain (TM), polyadenylation signal (pA), internal ribosomal entry site (IRES), placental alkaline phosphatase (PLAP), ventricle (V), and ribs (arrowhead) are shown. Bar, 200 μm (C). H&E, hematoxylin and eosin.