FIG. 10.

Clones of imaginal disk cells with increased Rbp1 expression have increased M1 retention. Results from whole-mount immunostaining of Drosophila larval imaginal disks are shown. An FLP-induced Gal4-positive clone expresses UAS-Rbp1 and is marked by the activity of a UAS-GFP transgene (A). The same cells (arrow) show enhanced expression of β-galactosidase from the M1 splicing reporter P{CZP-ORF3} relative to cells neighboring the clone (arrowhead) (B). For comparison, a GFP-positive clone expressing UAS-MycTra2 is shown (C) that similarly induces the reporter (D). In a parallel cross that omits the UAS-Rbp1 transgene, the GFP-expressing clones do not increase expression of the same reporter (E and F).