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. Author manuscript; available in PMC: 2007 Feb 16.
Published in final edited form as: Oncogene. 2006 Mar 30;25(14):2094–2104. doi: 10.1038/sj.onc.1209244

Figure 2.

Figure 2

Effect of HPV16 E7 siRNA on cell cycle-related gene expression from cervical cancer cells by transient transfection. HPV16 E7 siRNA or NS siRNA at 40 nM was transfected into HPV16+ cervical cancer cell lines, CaSki or SiHa and total cell RNA was prepared from each cell line after 24 hours of transfection. Approximate 30 μg of total cell RNA were hybridized to hCC-2 multi-probes (~ 4 X 106 CPM) and then subjected to RNase A/T1 digestion. RNA fragments protected were resolved in a 8% denaturing PAGE gel. Multi-probes undigested were run in parallel as controls and their corresponding protected products from total cell RNA were indicated by lines.