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. Author manuscript; available in PMC: 2007 Feb 16.
Published in final edited form as: Oncogene. 2006 Mar 30;25(14):2094–2104. doi: 10.1038/sj.onc.1209244

Figure 8.

Figure 8

Cells insensitive to endogenous siRNA are sensitive to exogenous siRNA duplexes. Transient transfection was used to compare E7 siRNA-insensitive cells, C-2 and D4SC subclone cells, with E7 siRNA–sensitive C-V cells, for their sensitivities to E7 siRNA 198 (a) at 1, 2, and 4 nM and p53 siRNA at 5 nM (b). The protein samples were prepared 24 hours after transfection and analyzed by Western blot for E7, p21, or p53. The same membrane was also examined by Western blot for β-tubulin as a control for sample loading.